Development of the automated faster acquisition technique for the observation of "the ultra-high voltage electron tomography"

本研究旨在开发“超高压电子层析成像”观测的自动快速采集技术，但常规的观测方式需要耗费大量精力和时间。因此，我们在运营支持系统中开发了以下项目；(1)一种新的自动对焦算法，(2)一种视场自动校正算法，(3)一种新的电子显微镜高速成像控制算法，(4)一种自动获取图像的时序控制算法。针对这种特殊的自动对焦算法，提出了一种从图像清晰度出发确定最优对焦条件的技术。我们使用两台专用于记录图像和控制图像的电子显微镜的摄像机，其摄像机针对每个任务进行了优化。因此，我们完成了自动化和减少时间的断层扫描观察。

This study is intended to develop the automated faster acquisition technique for the observation of "the ultra-high voltage electron tomography", however, the observation needs much effort and spends lots of time in the usual way. Therefore, we developed the following items in the operation support system; (1) A new algorithm for automatic focus (AF), (2) an automatic correction of field of view, (3) a new high-speed imaging for the control of electron microscope, (4) an automated sequence control for acquiring images. As regards this particular AF algorithm, the technique which an optimized focus condition was determined from the image sharpness, was newly developed. We use two cameras dedicated to recording images and to controlling the electron microscope from images, whose cameras are optimized for each task. As a result, we accomplished the automation and reduction in time for the tomography observation.