Constructing artificial rotors for rotary molecular motor F1(V1)-ATPase

F1(V1)- atp酶是atp驱动的旋转分子马达。针对所设计的转子能够旋转，我们研究了转子中哪些特定区域对旋转有贡献。突变体在转子轴和定子的接触区域内只留下了下面的一小部分，相对于野生型，突变体以一半的速度旋转，产生一半的旋转扭矩。结合以往的研究，转子中无残留物是旋转的必要条件。转子的一个基本性质可能只是棒状结构，或者转子的任何区域（残基）都可能对旋转有贡献。

F1(V1)-ATPase is an ATP-driven rotary molecular motor. In view of what designed rotor can rotate, we have investigated which specific regions in the rotor contribute for the rotation. The mutant, in which only the below slight portion was left within contact regions between rotor axle and stator, rotated at half speed and generated half rotational torque relative to wild-type. Together with previous studies, no residues in the rotor are essential for the rotation. An essential property of the rotor may be just the stick-like structure, or any regions (residues) of rotor may contribute for the rotation.