Analysis of peroxisome membrane biogenesis and application for Nano-medicine

Pex3p、Pex19p和Pex5p、Pex14p是过氧化酶体生物发生所必需的。推测Pex3p结合蛋白可以调节过氧化物体的膜蛋白插入到过氧化酶体中。为了鉴定结合蛋白，我们利用人的cDNA文库建立了酵母双杂交系统，发现了10种可能的蛋白。锥虫体内的糖体与人的过氧化物酶相似。糖体的生物发生受损对锥虫有致命的影响。作为对过氧化酶体生物发生学知识的应用，我们试图开发治疗锥虫病的化合物。我们建立了一个高通量的筛选系统来鉴定抑制Pex5p和Pex14p之间相互作用的化合物。利用这个筛选系统，我们开始筛选化学文库以寻找有效的化合物。

Pex3p, Pex19p and Pex5p, Pex14p are essential for the biogenesis of peroxisome. Putative Pex3p binding protein is suggested to regulate insertion of peroxisomal membrane proteins into the peroxisome. To identify the binding protein, we established yeast two-hybrid system using human cDNA library and found ten putative proteins.Trypanosome has glycosome that resembles to human peroxisome. Impaired biogenesis of glycosome is known to show fatal effect for trypanosome. As an application of the knowledge about biogenesis of peroxisome, we tried to develop the therapeutic compounds for trypanosomiasis. We established a high thoughput screening system to identify chemical compounds that inhibit the interaction between Pex5p and Pex14p. Using this screening system, we started to screen chemical libraries to find effective compounds.