The effect of EB virus infection in nasopharyngeal carsinoma cancer stem cell

我们试图从携带EB病毒的人鼻咽癌细胞系C666-1中建立EB病毒(EBV)阴性细胞系。C666-1每个细胞有大量的EBV拷贝，我们不能用药物治疗和稀释克隆的方法从C666-1建立EBV阴性细胞系。这些结果表明，EBV感染是维持C666-1细胞增殖所必需的。接下来，为了从鼻咽癌中分离出肿瘤干细胞群，我们分析了干细胞特异性转录因子SOX2在癌细胞系中的表达机制。SOX2启动子活性与SOX2基因表达水平相关，启动子阳性细胞群表现出较高的球状活性。此外，我们还鉴定了SOX2启动子结合蛋白，该蛋白通过调节SOX2启动子的活性来调控肿瘤干细胞的形成。

We had tried to establish EB virus (EBV) negative cell lines from EBV harboring human nasopharyngeal carcinoma cell line C666-1. C666-1 had a large number of EBV copies per cell and we could not establish EBV negative cell line from C666-1 using drug treatment and dilution cloning. These results suggest that EBV infection is essential to keep C666-1 cell proliferation. Next, to isolate cancer stem cell population from nasopharyngeal carcinoma, we analyzed the expression mechanism of stem cell specific transcription factor SOX2 in cancer cell lines. SOX2 promoter activity was related to SOX2 mRNA expression level and promoter positive cell population showed high sphere activity. Moreover, we identified SOX2 promoter binding protein and this protein regulated cancer stem cell formation by the regulation of SOX2 promoter activity.