A functional analysis of a secreted serine protease family for dermatophyte host invading mechanisms.

皮肤真菌是浅部真菌病的主要病原体。它们可能有独特的补偿性蛋白水解酶分泌机制，共同产生协同效应，导致感染。然而，由于分子遗传学研究中的显性选择标记的局限性，各种蛋白酶在其感染过程中的功能和作用仍不清楚。本研究的最终目的是阐明枯草杆菌蛋白(subtilisins，Subs)等大型蛋白酶基因家族在皮肤真菌入侵宿主中的功能和作用。为了实现这一点，我们研究了酵母FLP重组酶重组靶标(FRT)位点特异性重组系统在万氏关节皮病中的适用性。密码子优化的Flp/FRT介导的一步选择标记回收系统的成功开发，使得亚基因的顺序多重中断，促进了我们对这些真菌中单个基因的功能和网络的理解。

Dermatophytes are the major causative agents of superficial mycoses. They likely have unique compensatory protease secretion machinery that collectively produces synergetic effects, causing infection. However, because of the limitation in dominant selectable markers for their molecular genetics studies, functions and roles of each protease in their infection processes are still elusive.The final goal of this study was to elucidate functions and roles of large protease gene families, such as subtilisins (SUBs) in host invasion by dermatophytes. To attain this, we examined applicability of the yeast FLP recombinase-recombination target (FRT) site-specific recombination system in Arthroderma vanbreuseghemii. Successful development of the codon-optimized flp/FRT-mediated one-step selectable marker recycling system allowed the sequential multiple disruptions of SUB genes, advancing our understanding of functions and networks of individual genes in these fungi.