Development of evaluation of health effects and environmental carcinogens exposure in molecular epidemiology

多环芳烃（PAHs）是包含多种致癌物质的多种化合物。我们对钢铁厂工人（n=57）进行了一项横断面研究，以确定反映多环芳烃致癌性的生物标志物。采用气相色谱-质谱法测定15种多环芳烃的个人暴露水平。采用高效液相色谱法、荧光检测器和液相色谱-质谱联用分析尿液代谢物。检测了11种已知的尿代谢产物：1-，2-羟基萘，2-OH芴，1-,2-,3-，9-OH菲，1-OH芘，3-OH苯并[a]芘，6-OH芘，3-OH苯并[a]芘。总多环芳烃与尿2-OH萘的相关性最高。在检测到的尿液代谢物中，1-OH芘与“PAHs的Sigma致癌效能”（根据个人PAHs水平和相对效能因子计算出7种致癌PAHs的总和）相关性最大，表明尿1-OH芘是PAHs暴露最全面的致癌生物标志物。

Polycyclic aromatic hydrocarbons (PAHs) are multiple compounds which include many carcinogens. We conducted a cross-sectional study in steel plant workers (n=57), to identify biomarkers that reflect the carcinogenicity of PAHs. Level of personal exposure to 15 PAHs was measured using GC-MS. Urinary metabolites analyzed using HPLC with a fluorescence detector and LC-MSMS. Eleven known urinary metabolites 1-, 2-hydroxy(OH)naphthalene, 2-OH fluorene, 1-, 2-, 3-, 9-OH phenanthrene, 1-OH pyrene, 3-OH benz[a]anthracene, 6-OH chrysene, 3-OH benzo[a]pyrene were detected. The highest correlation was between Total-PAHs and urinary 2-OH naphthalene. Among the detected urinary metabolites, 1-OH pyrene was found to the greatest correlation with the "Sigma carcinogenic potency of PAHs" (sum of seven carcinogenic PAHs calculated from levels of personal PAHs and relative potency factors), indicated that urinary 1-OH pyrene was the most comprehensive carcinogenic biomarker of exposure to PAHs.