Development of the methods for the Diatom tests utilizing DNA binding ability of frustle

我们专注于硅藻的dna结合能力，它是由二氧化硅构成的，在混沌离子的存在下，试图为硅藻设计一个更好的法医测试。它被证明是可能捕获硅藻通过lambda DNA使用硅涂层磁珠，然后从磁珠分离和纯化。为了从血液中纯化硅藻，利用了硅藻囊在致乱剂存在下的DNA结合能力。程序与常用的血液DNA纯化方法基本相同，如蛋白酶K处理和变性剂。在这种条件下，DNA吸附到硅藻上，形成DNA/硅藻复合物。DNA/硅藻复合体采用简单的乙醇沉淀法回收，然后用DNA酶将DNA片段化。我们还开发并评估了一种利用二氧化硅的DNA结合能力染色硅藻的方法。

We have focused on the DNA-binding abilities of the diatom frustule, which is constructed of silica, in the presence of chaotropic ions, in an attempt to devise a better forensic test for diatoms. It is proved possible to capture diatoms via lambda DNA using silica-coated magnetic beads followed by isolation and purification from the Mag beads.For purification of diatoms from the blood, DNA binding ability of diatom frustule in the presence of chaotropic agent was utilized. The procedure is basically same as commonly used method for DNA purification from the blood as Proteinase K treatment and denaturation using chaotropic agent. Under this condition, the DNA adsorbed to the diatom and formed DNA/diatom complex. DNA/diatom complex was simply recovered ethanol precipitation and then the DNA was fragmented using DNase.we also developed and evaluated a method for staining diatoms that exploits the DNA binding ability of silica.