The role of Dok adaptors in pulmonary disorders

Dok衔接蛋白在酪氨酸激酶介导的信号通路调控中发挥重要作用。在这项研究中，我特别研究了Dok适配器在哮喘等肺部疾病中的作用。虽然有许多引起哮喘的因素，但免疫细胞功能障碍是该病的主要原因之一。在这些免疫细胞中，可以检测到三个Dok接头，因此，使用敲除小鼠研究了它们在哮喘中的作用。正如预期的那样，缺乏三个Dok接头的小鼠表现出气道高反应性的异常增加，这是哮喘的一种生物学指标。与先前的发现一致，这些小鼠支气管肺泡灌洗液中Th2细胞因子的水平也升高。组织学检查显示气道上皮细胞增生，杯状细胞增多。这些结果证明Dok衔接蛋白在哮喘的调控中起关键作用。

Dok adaptor proteins play important roles on the proper regulation of tyrosine kinase-mediated signaling pathways. In this study, I particularly investigated the role of Dok adaptors in pulmonary disorders such as asthma. Although many causative factors of asthma have been suggested, dysfunction of immune cells is one of the major reason of this disease. In these immune cells, three Dok adaptors can be detected, therefore, the role of them in asthma was investigated using knockout mice.As expected, mice lacking three Dok adaptors exhibited an abnormal increase in airway hyperreactivity, a biological measure of asthma. Consistent with previous findings, the levels of Th2 cytokines in bronchoalveolar lavage fluids were also elevated in these mice. Furthermore, histological examination revealed hyperplasia of airway epithelial cells and accumulation of goblet cells. These results proved that Dok adaptor proteins play a key role on the regulation of asthma.