The role of proteoglycans in the modulation of EGFR family and it's ligand in lung fibrosis

特发性肺纤维化是一种进行性疾病，临床预后较差。然而，治疗策略尚未建立。在本研究中，我们检测了表皮生长因子受体（EGFR）及其配体在博莱霉素诱导肺纤维化小鼠离体肺中的表达。我们将C56BL6和Balb/c进行了比较，Balb/c是一种已知在博莱霉素作用下表现出较强纤维化变化和轻度纤维化变化的菌株。同时评估蛋白多糖的表达，目的是探索与EGFR和EGFR配体的关系。与Balb/c小鼠相比，C56BL6小鼠的肺部出现明显的纤维化变化，胶原蛋白含量更高。与Balb/c小鼠相比，C56BL6中amphiregulin、epiregulin、versican和decorin的表达似乎较低，而EGFR在C56BL6中似乎高表达。这些发现为进一步的研究提供了基础。

Idiopathic Pulmonary Fibrosis is a progressive disease with poor clinical outcome. And yet treatment strategy has not been well-established. In this study we examined the expression of Epidermal Growth Factor Receptors (EGFR) and it`s ligands in isolated lungs from mouse model of bleomycin-induced pulmonary fibrosis. We compared C56BL6 and Balb/c, a strain known to show stronger fibrotic change and mild fibrotic change with bleomycin, respectively. Proteoglycan expression was concomitantly evaluated with intention to explore the association with EGFR and EGFR ligands. Lungs from C56BL6 mice appeared to have apparent fibrotic changes and higher collagen content compared to Balb/c mice. Expression of amphiregulin, epiregulin, versican and decorin seemed to be lower in C56BL6 compared Balb/c mice, whilst EGFR seemed to be highly expressed in C56BL6. These findings provide bases for further investigation.