Re-uniting marsupials and eutherians by embryonic micromanipulation

将生命代代相传的独特责任通常取决于配子。该项目将使用新的生殖技术来研究重新编程体细胞核时卵母细胞的特性，并将使用有袋类和真核生物的体细胞核来测试这一点。我们还将利用有袋类和真兽类的基因插入卵母细胞，创造出第一种转基因有袋类动物。我们还将通过卵胞浆内单精子注射(ICSI)来研究遗传距离越来越远的物种的精子受精有袋类卵子的能力。

The unique responsibility for transmitting life from generation to generation normally depends on the gametes. This project will use new reproductive technologies to investigate the properties of the oocyte in reprogramming somatic cell nuclei, and will use the nuclei of both marsupial and eutherian somatic cells to test this. We will also use both marsupial and eutherian genes to insert into the oocyte to create the first transgenic marsupials. We will also investigate the ability of spermatozoa from species of increasing genetic distance to ferttilise marsupial eggs using intracytoplasmic sperm injection (ICSI).