Development of novel diagnostic and therapeutic biomarkers for hypereosinophilic syndrome

高嗜酸性粒细胞综合征(HES)是一种以原因不明的长期嗜酸性粒细胞增多为特征的异质性疾病。我们明确了参与研究的患者的临床鉴别诊断，并通过RT-PCR检测了FIP1L1-PDGFRA嵌合mRNA的存在。此外，我们还使用从不同患者的外周血中提取的磷脂肽进行了磷酸蛋白质组学研究(NanoLC-MS/MS)，发现与嗜酸性粒细胞增多症的潜在状况相关的不同图谱。这一信息有助于HES的鉴别诊断。

Hypereosinophilic syndrome (HES) is a heterogeneous disorders characterized by prolonged eosinophilia with causes unknown. We clarified the clinical differential diagnosis of the patients who participated in this study, and tested for the existence of FIP1L1-PDGFRA chimeric mRNA by RT-PCR. Moreover, we also performed phosphoproteomic studies by mass spectrometry (nanoLC-MS/MS) using the phsphopeptides extracted from peripheral blood of various patients and found the different profiles with which correlate the underlying condition of eosinophilia. This information of phosphopeptides could be useful in the differential diagnosis of HES.