Negative regulation of TLR signaling by G-CSF and ATP in leukocytes

多种toll样受体在人白细胞（如中性粒细胞和单核细胞）上表达，在先天免疫中起重要作用。虽然toll样受体介导的细胞活化的分子机制已经被越来越多地了解，但该系统的正调控和负调控机制仍然是难以捉摸的。阐明生理分子的负调控机制对于炎症和组织损伤的可能治疗尤为重要。我们发现该系统受粒细胞-巨噬细胞集落刺激因子、干扰素- α和干扰素- γ的正调控，受粒细胞集落刺激因子和ATP（三磷酸腺苷）的负调控。

Various Toll-like receptors are expressed on human leukocytes such as neutrophils and monocytes, and play an important role in the innate immunity. Although the molecular mechanisms of Toll-like receptor-mediated cell activation have been increasingly understood, the positive and negative regulatory mechanisms on this system remain to be elusive. To elucidate the negative regulatory mechanisms by physiological molecules is particularly important for possible treatment of inflammation and tissue damage. We found that this system was positively regulated by granulocyte-macrophage colony-stimulating factor, interferon-alpha and interferon-gamma, and was negatively regulated by granulocyte colony-stimulating factor and ATP (adenosine tri-phosphate).