Role of Na/Ca exchanger on vascular tone of fetal and neonatal ductus arteriosus and peripheral pulmonary artery

(1)各亚型Na/Ca交换元件(SLC8A1、A2、A3)均见于肺动脉和动脉导管。A1和A3表达水平较高，而A2表达水平较低。(2)在发育性变化中，动脉导管和肺动脉的SLC8A1有轻微下降的趋势，而在心室肌中有明显的变化。另一方面，SLC8A3有随发育而增加的趋势。免疫组织化学染色显示动脉导管和肺动脉中A3强阳性，A1次之。SLC8A2免疫组化染色为阴性。(4)将SLC8A1基因导入HEK293细胞。(5)用膜片钳方法测量了SLC8A1的Na/Ca交换电流。然而，在各种条件下，如缺氧，电流的测量还没有完成。研究将继续进行。

(1) All isoform of Na/Ca exchanger member (Slc8A1, A2, A3) was seen in pulmonary artery and ductus arterisus. High expression levels were observed in A1 and A3, whereas A2 expression level was low. (2) In developmental changes, Slc8A1 tends to decrease slightly in ductus arteriosus and pulmonary artery, compare with significant change is observed in ventricular muscle. On the other hand, Slc8A3 tends to increase with development. In immunostaining analyses showed strong stain in A3 followed in A1 in ductus arteriosus and pulmonary artery. Slc8A2 showed negative immunostaining. (4) Transfection of Slc8A1 into HEK293 cell was performed. (5) Measurement of the Na/Ca exchanger current of Slc8A1 was performed by patch clamp method. However, measurements of the currents under various conditions such as hypoxia were not completed. Research will be continued.