Basic and clinical studies regarding to the identification of inflammatory factors promoting the malignant transformation mechanism in digestive organ cancer

为探讨人肝细胞癌的癌变过程，分析了人肝细胞癌组织巨噬细胞()对HMGB1及其受体TLR4表达的影响。此外，还研究了FRB免疫毒素对是否具有抑制肿瘤恶性转化的作用。在肝细胞癌中，血清HMGB1水平与数目、数目与患者预后相关。对于EMT的相关知识，转录因子ZEB-1也参与其中。另一方面，将FRB免疫毒素皮下注射给裸鼠HepG2移植瘤模型后，有明显的促瘤作用。综上所述，我们认为HMGB1及其衍生的可能参与了肝癌的恶性转化，FRB免疫毒素可能是一种治疗策略。

To investigate the cancer transformation of human hepatocellular carcinoma (HCC), the change of HMGB1 and its receptor TLR-4 with tissue macrophage (TAM) were analyzed. Moreover, the usefulness of FrB immunotoxin against TAM was studied if it could diminish tumor's malignant transformation. In HCC, correlations of HMGB1 level and number of TAM, and the number of TAM and patients prognosis were seen. For the knowledge about EMT, transcription factor ZEB-1 participated, too. On the other hand, after giving FrB immunotoxin to nude mouse HepG2 transplant model under skin, a clear effect of tumor promotion was seen. With these considerations, it was suggested that HMGB1 and derived TAM would contribute to the malignant transformation of HCCs, and FrB immunotoxin could be a treatment strategy.