Fundamental research on identification and its control of the priming factor in liver cancer proliferation

肝癌增殖启动因子的识别及其调控基础研究

• 批准号: 14370359
• 负责人: UENO Shinichi
• 金额: $ 3.52万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14370359/
• 关键词: hepatocarcinogenesis; TNFa; IL8; ROS; roxithromicin; 肝細胞癌; IL8; TNF; Roxithromycin; ROS; IL6; IL-6; TNF-a; 1L-8

1)In 42 human HCC samples, IL-8 expression and its biological and clinical significance was studied. Using RT-PCR to IL-8 mRNA, 32 HCC samples were positive of IL-8. IL-8 expression of tumor tissue was not correlated with its angiogenesis studied by CD34 immunostaining. Hence, the incidence of vessel involvement was much higher in IL-8 positive group than in IL-8 negative group (n=10). Thus, TNM staging of HCC in positive group was higher than that in negative group. Overall, it is concluded that IL-8 expression in HCC may affect tumor cell behavior more than angiogenesis.2)In rat-hepatocrcinogenesis induced by Diethylnitrosamin (DEN), liver weight and cancer volume were increased at 17-week DEN administration. Moreover, tissue MDA level was increased, and tissue NF-κB activation and the increase of iNOS level were shown. TNP470 (50mg/kg) and Roxithromicin (RXM 100mg/kg) inhibited the NF-κB activation and the following iNOS and MDA level.3)By using nude mouse- dorsal bag model, it is shown that RXM inhibited tumor angiogenesis of HepG2.

1)在42个人类HCC样本中，研究了IL-8的表达及其生物学和临床意义。使用 RT-PCR 对 IL-8 mRNA 进行检测，32 个 HCC 样本呈 IL-8 阳性。 CD34免疫染色研究肿瘤组织的IL-8表达与其血管生成不相关。因此，IL-8阳性组血管受累的发生率远高于IL-8阴性组（n=10）。因此，阳性组HCC的TNM分期高于阴性组。总体而言，得出的结论是，HCC中IL-8表达对肿瘤细胞行为的影响可能大于对血管生成的影响。2)在二乙基亚硝胺(DEN)诱导的大鼠肝细胞癌变中，给予DEN 17周后，肝脏重量和肿瘤体积增加。此外，组织MDA水平增加，组织NF-κB活化和iNOS水平增加。 TNP470(50mg/kg)和罗红霉素(RXM 100mg/kg)抑制NF-κB活化以及随后的iNOS和MDA水平。3)通过裸鼠背袋模型，显示RXM抑制HepG2的肿瘤血管生成。

项目成果

Roxithromycin inhibits constitutive activation of nuclear factor {kappa}B by deminishing oxidative stress in a rat model of hepatocellular carcinoma

罗红霉素通过减少肝细胞癌大鼠模型中的氧化应激来抑制核因子 {kappa}B 的组成性激活

• 发表时间: 2005
• 期刊: Clin Cancer Res 11(15):
• 作者: Ueno S;Aoki D;Kudo F;Hiwatashi K;Matsushita K;Oyama T;Maruyama I;Aikou T.
• 通讯作者: Aikou T.

Roxithromycin inhibits constitutive activation of nuclear factor {kappa}B by diminishing oxidative stress in a rat model of hepatocellular carcinoma

罗红霉素通过减少肝细胞癌大鼠模型的氧化应激来抑制核因子 {kappa}B 的组成性激活

• 发表时间: 2005
• 期刊: Clin Cancer Res. 11(15)
• 作者: Ueno S;Aoki D;Kubo F;Hiwatashi K;Matsushita K;Oyama T;Maruyama I;Aikou T.
• 通讯作者: Aikou T.

Roxithromycin inhibits angiogenesis of human hepatoma cells in vivo by suppressing VEGF production.

Roxithromycin 通过抑制 VEGF 的产生来抑制体内人肝癌细胞的血管生成。

• 发表时间: 2005
• 期刊: Anticancer Res 25(1A)
• 作者: Aoki D;Ueno S;Kubo F;Oyama T;Sakura T;Matsushita K;Maruyama I;Aikou T.
• 通讯作者: Aikou T.

Roxithromycin inhibits constitutive activation of nuclear factor {kappa}B by diminishing oxidative stress in a rat model of hepatocellular carcinoma.

Roxithromycin 通过减少肝细胞癌大鼠模型中的氧化应激来抑制核因子 {kappa}B 的组成性激活。

• 发表时间: 2005
• 期刊: Clin Cancer Res 11(15)
• 作者: Ueno S;Kubo F;et al.
• 通讯作者: et al.

Interleukin 8 in human hepatocellular carcinoma correlates with cancer cell invasion of vessels but not with tumor angiogenesis

• DOI: 10.1245/aso.2005.07.015
• 发表时间: 2005-10-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Kubo, F;Ueno, S;Aikou, T
• 通讯作者: Aikou, T