A novel urinary marker for detection of urothelial carcinoma The clinical significance and the biological role of 80KDa glycoprotein

对约100例膀胱癌患者尿液进行Western blot分析，利用抗体对80KDa糖蛋白（我们之前检测过）产生反应。结果显示，这些80KDa糖蛋白的表达水平在晚期病理级病例中较高，具有统计学意义。实时荧光定量PCR结果显示，80KDa糖蛋白mRNA水平在正常尿路上皮细胞系、低级别尿路上皮癌细胞系和高级别尿路上皮癌细胞系中依次较高。组蛋白去乙酰化酶抑制剂和DNA甲基化抑制剂治疗可恢复uthelial癌细胞LTF的表达。在根治性膀胱切除术标本中，癌细胞和正常尿路上皮细胞之间80KDa糖蛋白的免疫反应性没有区别。

Western blot analysis was performed using urine of the patients with bladder cancer (about 100 cases) by using the antibody reacts to the 80KDa glycoprotein (we detected previously). The result was revealed that these expression levels of 80KDa glycoprotein were higher in the advanced pathological grade cases statistifically significance.The real-time PCR revealed that mRNA level of 80KDa glycoprotein was higher in the order of normal urothelial cell line, low-grade urothelial cancer cell line and high-grade urothelial cancer cell lines.Treatment with histone deacetylase inhibitor and DNA methylation inhibitor restored LTF expression in utohelial cancer cell lines. Immunoreactivity of the 80KDa glycoprotein was undistinguished between cancer cells and normal urothelial cells in radical cystectomy specimens.