Identification and functional analysis of cytokine signal transduction-related factors in human urethral rhabdosphincter

我们探索了人RS中特异性表达的因子，并研究了这些因子对RS卫星细胞生长、分化的影响以及细胞内信号转导机制。免疫组化显示RS细胞外基质中TGF-β表达显著增加。此外，我们还发现TGF-β可以通过Smad 2/3磷酸化抑制人RS卫星细胞的增殖和分化。在大鼠实验中，随着生育后年龄的增长，尿道功能显著下降，人们认为生育后年龄的增长减弱了尿道的支持。另一方面，随着年龄的增长，尿道ECM的转录量显著减少，而炎性细胞因子的转录量在青年组和老年组之间没有显著变化。随着年龄的增长，ECM减少或尿道慢性炎症状态包括RS可能减弱尿道支持系统，并可能是老年人SUI的一部分。

We explored the identification of specifically expressed factors in human RS and examined the effect on RS satellite cell growth, differentiation and the mechanisms of intracellular signal transduction by those factors. TGF-beta highly increased in ECM of RS by immunohistochemistry. Moreover, we identified that TGF-beta could inhibit cell proliferation and differentiation of human RS satellite cells through Smad2/3 phosphorylation.In the experiments using rat, urethral fnctions significantly decreased with aging after child-birth, and it has been considered that aging after child-birth attenuated urethral support. On the other hand, transcripts of ECM in urethra were significantly decreased with aging, although there were no significant changes in those of inflammatory cytokines between young and aged groups. Reduction of ECM or chronic inflammatory state in urethra including RS with aging might attenuate urethral support system,and be a part of SUI in elderly people.