Development of ocular irritancy test using cultured corneal epithelial sheet

活体Draize眼科测试已经成为眼睛刺激的国际标准测试，经常因为伦理和科学原因而受到批评。化妆品指令的第7条修正案将禁止对化妆品成分进行动物试验。因此，替代策略是必要的，允许在类似于体内暴露的条件下测试具有广泛物理化学性质的化学物质。我们最近通过使用分层培养的角膜上皮创建了一个比传统模型更接近活体的细胞损伤修复实验模型。MFLX眼用液组和LVFX眼用液组角膜上皮损伤修复程度与阴性对照组相近。此外，我们还开发了更方便的测试，使我们能够通过PUT眼刺激测试进行高强度测试。

The in vivo Draize eye test, which has become the international standard assay for eye irritation is often criticized for both ethical and scientific reasons. The 7th amendment of the Cosmetic directive will lead to the ban of animal testing for cosmetic ingredients. Thus, alternative strategies are necessary which allow the testing of chemicals with wide physicochemical properties under conditions similar to in vivo exposure.We recently created an experimental model of cell damage repair closer to the live body than conventional models, by using layered sheets of cultured corneal epithelium. The corneal epithelial defect was repaired in the ophthalmic MFLX solution treatment and the ophthalmic LVFX solution treatment to a degree similar to that in the negative control group. Furthermore, we have developed more convenient test that enable us to perform high through put eye irritancy test.