A study of tissue trauma with hemorrhagic shock induced remote organ failure.

本研究旨在探讨软组织外伤后多器官功能衰竭的发生机制。首先，我们制作了组织创伤动物模型，并验证了该模型用于创伤研究的有效性。我们制作的创伤模型是一种非镇痛性的小鼠组织创伤模型，该模型是通过精确调整体重的切碎的供体同种组织骨基质（TBX）背侧皮下植入构建的，并在21小时后进行研究。接下来，我们使用我们的生产模式研究了两击现象（组织创伤/败血症）对胃肠道运动的影响。同基因TBX模型是一种高度可控、可靠的组织损伤存活模型，只有在组织损伤/脓毒症联合模型中，胃肠运动才发生严重改变，因此出现了两击协同作用。

The aim of our study was to investigate the mechanism of multiple organ failure after soft tissue trauma. First, we produced an animal model of tissue trauma, and verified the validity of the model for the study of trauma. Our produced trauma model was a non-analgesic murine tissue trauma model, which was constructed by the dorsal subcutaneous implantation of minced donor syngeneic tissue-bone matrix (TBX) precisely adjusted to body weight and studyed after 21 hrs. Next, we investigated the effect of two-hit phenomenon (tissue trauma/sepsis) on gastrointestinal motility using our produced mode. Syngeneic TBX model was a highly controllable and reliable survival model of tissue injury, and two hit synergy occurred as gastrointestinal motility was only severely altered in the combined tissue trauma/sepsis model.