Genome sequence analysis of biofilm-forming Streptococcus constellatus

我们从根尖脓肿病灶中分离出星座链球菌H39株。扫描电子显微照片显示菌株H39在细胞周围具有致密的网状结构，这是产胞外多糖细菌的特征。菌株H39在流动室中生长形成生物膜。我们报道了H39菌株的基因组测序草图，可用于进一步的生物膜形成机制的遗传研究。通过焦磷酸测序进行基因组测序。重叠群之间的推定缺口通过对缺口上的聚合酶链反应扩增子进行测序来闭合。高质量的基因组测序结果表明，H39菌株含有3个核糖体RNA基因、45个转运RNA基因和1,926个蛋白质编码基因。我们还发现菌株H39具有编码胞外多糖合成蛋白和ABC型多糖转运蛋白的基因。这些基因可能与S.星座。

We isolated Streptococcus constellatus strain H39 from an apical abscess lesion. Scanning electron micrographs revealed that strain H39 had dense meshwork-like structures around the cells, which are a characteristic of exopolysaccharide-producing bacteria. Strain H39 grown in flow chambers formed biofilm. We reported a draft genome sequencing of strain H39 which can be used for further genetic study on biofilm formation mechanisms. The genome sequencing was performed by pyrosequencing. The putative gaps between contigs were closed by sequencing of polymerase chain reaction amplicons over the gaps. A high-quality draft genome sequence indicated that strain H39 had 3 ribosomal RNA genes, 45 transfer RNA genes and 1,926 putative protein-coding genes. We also found that strain H39 possessed genes encoding exopolysaccharide biosynthesis proteins and ABC-type polysaccharide transport proteins. These genes might contribute to the biofilm formation of S. constellatus.