Studies on the development of the periodontal examination by using saliva

唾液牙周检查技术的发展研究

• 批准号: 12357014
• 负责人: KURIHARA Hidemi
• 金额: $ 27.56万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12357014/
• 关键词: saliva; periodontal examination; outer membrane protein; Streptococcus anginosus; diabetes; matrix metalloproteinase-8; 8-hydroxy-deoxyguanosine; sulfated glycosaminoglycan; ストレプトコッカス アンギノサス; 硫酸化グコサミノグルカン; Porphyromonas gingivalis; Actinobacill

In this project, we analyzed the development of the periodontal examination by using saliva from the diversified aspects. There were products of IgA antibody against Porphyromonas gingivalis (P.g) in saliva from patients with progressive periodontitis. And it can be possible to identify the P.g infection by measurement of specific IgA antibody in saliva. We demonstrated the detection method of 9 putative periodontopathic bacteria in saliva by polymerase chain reaction (PCR). IgA in saliva from periodontal patients, which serum strongly react outer membrane protein (Omp) from Actiobacillus actiomycetemcomitans (A.a), reacted A.a-Omp antigen in enzyme-linked immunosorbent assay (ELISA). And this method enable us to identify the infection of A.a. We established the specific PCR-detection method of Streptococcus anginosus (S.a), and revealed the unique relationship between S.a and antibacterial peptide (hBD-2) from epitherial cells. And a part of molecular mechanism of S.a pathogenicity wa … More s clear. We found that it was available to use a diabetic patient model, which was improved the insulin resistance and control level of Blood sugar by periodontal treatment, to develop the periodontal examination by using saliva. There was no difference between diabetic group with periodontal disease and that without periodontal disease in the point of biochemical examination data by using saliva, so we could reveal no relevance between diabetes and periodontitis. To examine the elastase activity and the expression of active oxygen in neutrophil from saliva, it was useful to realize the state of periodontal disease and to judge the risk of that. By measuring the level of active form of matrix metalloproteinase-8 (MMP-8) in saliva, we can select the patient with active type periodontitis. The mean value of 8-hydroxy-deoxyguanosine (8-OHdG) in saliva of periodontal patients was higher than that of normal subjects. So 8-OHdG levels in saliva appear to reflect the status of periodontal health. Sulfated glycosaminoglycan (S-GAG), which was indicated as the good marker of alveolar bone metabolism, couldn't detect in saliva from the subjects with gingivitis Less

本课题从多方面分析了唾液牙周检查的发展。进行性牙周炎患者唾液中存在抗牙龈卟啉单胞菌伊加抗体产物。通过唾液中特异性伊加抗体的检测，可用于诊断P.g感染。我们建立了聚合酶链反应（PCR）检测唾液中9种牙周致病菌的方法。用酶联免疫吸附试验（ELISA）检测牙周病患者唾液中伊加与伴动放线杆菌（A. a）外膜蛋白（Omp）的反应性。这种方法使我们能够鉴定A.a.建立了咽峡炎链球菌（Streptococcus anginosus，S.a）的特异性PCR检测方法，并揭示了S.a与肠上皮细胞抗菌肽（hBD-2）的独特关系。并对S.a致病的部分分子机制进行了初步探讨。 ...更多信息 安全。我们发现，使用糖尿病患者模型，通过牙周治疗改善胰岛素抵抗和血糖控制水平，可以利用唾液进行牙周检查。糖尿病合并牙周病组与无牙周病组唾液生化指标无差异，提示糖尿病与牙周炎无相关性。检测唾液中性粒细胞中弹性蛋白酶活性和活性氧的表达，有助于了解牙周病的病情，判断牙周病的危险性。通过检测唾液中活性型基质金属蛋白酶-8（MMP-8）的水平，可以筛选出活动型牙周炎患者。牙周病患者唾液中8-羟基脱氧鸟苷（8-OHdG）含量高于正常人。因此，唾液中8-OHdG水平似乎反映了牙周健康状况。硫酸化糖胺聚糖（S-GAG）是反映牙槽骨代谢的良好标志物，但在牙龈炎患者的唾液中检测不到S-GAG。

项目成果

Takeuchi, Y.: "Treponema socranskii, Treponema denticola, and Porphyromonas gingivalis are associated with severity of periodontal tissue destruction"J Periodontol. 72. 1354-1363 (2001)

Takeuchi, Y.：“索氏密螺旋体、齿状密螺旋体和牙龈卟啉单胞菌与牙周组织破坏的严重程度有关”《牙周病杂志》。

Nomura, Y.: "Mutans streptococci and Lactobacillus as risk factors for dental caries in 12-year-old children"Jpn J Infect Dis. 54. 43-45 (2001)

Nomura, Y.：“变形链球菌和乳酸菌是 12 岁儿童龋齿的危险因素”Jpn J Infect Dis。

Senpuku, H.: "Inhibitory effects of MoAbs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of Streptococcus mutans"Scand J Immunol. 54. 109-116 (2001)

Senpuku, H.：“MoAb 对表面蛋白抗原在变形链球菌体外实时粘附和体内再定殖中的抑制作用”Scand J 免疫学。

Sakamoto, M. et al.: "Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA Libraries"Microbiol Immunol. 44. 643-652 (2000)

Sakamoto, M. 等人：“通过 16S rDNA 文库序列分析比较健康受试者和两名牙周炎患者唾液中的口腔细菌菌群”Microbiol Immunol。

Simizu, A. et al.: "Supportive Periodontal Treatment期にある歯周病患者の根面う蝕発症の実態"岡山歯学会雑誌. 20. 119-127 (2001)

Simizu, A. 等人：“牙周病患者牙周支持治疗阶段根龋发展的实际状况”冈山牙科学会杂志 20. 119-127 (2001)。