Studies on the development of the periodontal examination by using saliva

在本项目中，我们从多个方面分析了唾液牙周检查的发展。进行性牙周炎患者唾液中有抗牙龈卟啉单胞菌(P.G)IgA抗体产物。通过检测唾液中的特异性IgA抗体，可以对P.G感染进行鉴定。应用聚合酶链式反应(PCR)检测唾液中9种疑似牙周病细菌。应用酶联免疫吸附试验(EL ISA)检测牙周患者唾液中与伴生放线杆菌外膜蛋白(OMP)强反应的IGA。这种方法使我们能够识别A.A.的感染。建立了链球菌(S.A)特异的PCR检测方法，揭示了S.A与外胚层细胞中抗菌肽(HBD-2)的独特关系。沙门氏菌…致病的部分分子机制S更清楚了。我们发现，通过牙周治疗改善胰岛素抵抗和血糖控制水平的糖尿病患者模型，利用唾液进行牙周检查是可行的。糖尿病伴牙周病组与无牙周病组唾液生化检测数据无明显差异，提示糖尿病与牙周炎无相关性。检测唾液中中性粒细胞弹性蛋白酶活性和活性氧的表达，有助于了解牙周疾病的状态，判断牙周疾病的危险性。通过检测唾液中基质金属蛋白酶-8(MMP8)活性形式的水平，可以筛选出活动型牙周炎患者。牙周病患者唾液中8-羟基脱氧鸟苷(8-OHdG)的平均值高于正常人。因此，唾液中8-OHdG水平似乎反映了牙周健康的状况。被认为是良好的牙槽骨代谢标志物的S糖胺聚糖(GAG)在牙周炎较少的受试者唾液中检测不到

In this project, we analyzed the development of the periodontal examination by using saliva from the diversified aspects. There were products of IgA antibody against Porphyromonas gingivalis (P.g) in saliva from patients with progressive periodontitis. And it can be possible to identify the P.g infection by measurement of specific IgA antibody in saliva. We demonstrated the detection method of 9 putative periodontopathic bacteria in saliva by polymerase chain reaction (PCR). IgA in saliva from periodontal patients, which serum strongly react outer membrane protein (Omp) from Actiobacillus actiomycetemcomitans (A.a), reacted A.a-Omp antigen in enzyme-linked immunosorbent assay (ELISA). And this method enable us to identify the infection of A.a. We established the specific PCR-detection method of Streptococcus anginosus (S.a), and revealed the unique relationship between S.a and antibacterial peptide (hBD-2) from epitherial cells. And a part of molecular mechanism of S.a pathogenicity wa … More s clear. We found that it was available to use a diabetic patient model, which was improved the insulin resistance and control level of Blood sugar by periodontal treatment, to develop the periodontal examination by using saliva. There was no difference between diabetic group with periodontal disease and that without periodontal disease in the point of biochemical examination data by using saliva, so we could reveal no relevance between diabetes and periodontitis. To examine the elastase activity and the expression of active oxygen in neutrophil from saliva, it was useful to realize the state of periodontal disease and to judge the risk of that. By measuring the level of active form of matrix metalloproteinase-8 (MMP-8) in saliva, we can select the patient with active type periodontitis. The mean value of 8-hydroxy-deoxyguanosine (8-OHdG) in saliva of periodontal patients was higher than that of normal subjects. So 8-OHdG levels in saliva appear to reflect the status of periodontal health. Sulfated glycosaminoglycan (S-GAG), which was indicated as the good marker of alveolar bone metabolism, couldn't detect in saliva from the subjects with gingivitis Less