A basic study of long-term storage of experimental animal tissue and organ with voltage cryopreservation method.

我们对器官或组织的高压冷冻保存方法进行了研究。在实验中，使用了小鼠的卵巢或睾丸。蛋黄也被冻住了。将小鼠的器官用5000 V的电压冷却至-15或-30 ℃，然后置于液氮中进行冷冻保存。为了解冻，将器官置于室温下并施加5，000 V的电压。解冻后将睾丸或卵巢移植到受体体内。不可能从卵巢或冷冻保存的睾丸中获得配子。然而，该组织的一部分还活着。解冻后，含有低温不可逆蛋白质的蛋黄仍待包装。本研究结果提示了器官保存的可能性。但是，不能保存完整的性状，在今后的器官保存实验中，有必要对细胞内物质的保护进行研究。

We have studied the high voltage cryopreservation method of organ or tissue. In the experiment, used the ovaries or testes of the mouse. And egg yolk was also frozen. The organs of mice were cooled to -15 or -30 degree C with application of 5000 V, and then placed in liquid nitrogen for cryopreservation. For thawing, the organs were placed at room temperature and a voltage of 5,000 V was applied. Testes or ovaries were transplanted into the recipient after thawing. It was not possible to obtain gametes from the ovaries or testes were cryopreserved. However, part of the organization were alive. After thawing, egg yolk that contains the irreversible protein at low temperature remained to be packed. Results of this study, the possibility of organ preservation has been suggested. However, can not preservation of complete traits.The experiments of organ preservation in the future, it is necessary to study the protection of intracellular material.