Bioimaging technique to observe skeletal muscle metabolites in vivo

采用体内生物成像模型和显微注射技术，通过显微注射将低pH溶液加载到单纤维中，测试了细胞内的稳态。将成年雄性Wistar大鼠完整的斜方棘肌外化，用荧光探针BCECF-AM负载。用微注射法将低pH溶液注入肌肉。该模型提供了一个独特的机会来研究在接近生理条件下pH对肌肉缓冲动力学的实验操作的后果

Using an in vivo bioimaging model and microinjection technique, we tested homeostasis of intracellular when low pH solution was loaded in single fiber by microinjection. The intact spinotrapezius muscle of adult male Wistar rats was exteriorized and loaded with the fluorescent probe BCECF-AM. Muscle was loaded low pH solution by microinjection. This model offers a unique opportunity to investigate the consequences of experimental manipulations of pH on muscle buffering dynamics under close-to-physiological conditions