Total analysis of adhesive protein genes from mussels.

本研究对海水、淡水贻贝和一种水生昆虫的粘附蛋白基因进行了克隆。 在淡水贻贝的情况下，Limnoperna fortunei，fp-2样蛋白基因被发现作为一个完整的序列。 一种酶，酪氨酸酶，也被认为是在贻贝脚表达。 结果表明，淡水贻贝的蛋白质聚集体中存在较高水平的Asp，说明静电相互作用是淡水环境中蛋白质聚集的重要动力之一，而DOPA的存在是保守的。 这种水生昆虫有一种巨大的多肽，可以用来形成胶结物和丝。 丝/骨水泥蛋白具有高水平的磷酸化丝氨酸残基，并且Ca 2+与磷酸化蛋白之间的相互作用将驱动骨水泥/丝的形成。

Gene cloning of adhesive proteins from marine and freshwater mussels and one species of aquatic insect have been investigated. In the case of freshwater mussel, Limnoperna fortunei, a fp-2-like protein gene was found as an entire sequence. An enzyme, tyrosinase, was also suggested to be expressed in the mussel foot. The results indicated that freshwater mussels adopts the fp-like protein having higer level os Asp, which imply that electrostatic interactions is one of significant force for protein aggregation in freshwater environments, while presence of DOPA is conserved. The aquatic insect have a giant polypeptide for cement and silk formation. The silk/cement protein has high level of phosphorylated serine residues, and interaction between Ca2+ and the phosphorylated proteins will drive the cement/silk formation.