Development of the novel chemoselective capture method for sphingolipids

为了建立对鞘氨醇的化学选择性捕获的新方法，进行了目标分子的大规模有机合成。从D-和L-丝氨酸制备具有四种不同立体化学的四种标准样品。选择戊二醛基团作为官能团，以化学选择性地与2-氨基-1，3-二醇官能团反应。合成了苯基戊二醛衍生物，并将其连接到树脂表面。红外光谱证实了固定化效果，OPA衍生化法检测鞘氨醇的结果显示了其捕集效果。为了检测血清中的少量鞘氨醇，利用先前合成的标准样品成功地进行了这种新方法的小型化。

To create new methodology of chemoselective catch toward sphingosine, large scale organic synthesis of target molecule was performed. Four standard samples having four different stereochemistries were prepared from D- and L-serine. The glutaraldehyde group was selected as functional group to react chemoselectively toward an 2-amino-1,3-diol function. The phenyl glutaraldehyde derivative was synthesized and connected with surface of the resin. The immobilization efficiency was confirmed by IR. The OPA derivatization method for detection of sphingosines reveals clearly its efficiency of the catching. To detect small amount of sphingosine in serum, downsizing of this new methodology was successfully performed utilizing the previously synthesize standard samples.