Development of Fluorescent Probe for Visualization of Intracellular Chloride Anion

我们研制了一种新型的吖啶酰胺型氯离子荧光传感器。该探针在生物相关浓度范围内(从1 mM到100 mM)，基于碰撞猝灭机制，对氯离子显示出较大的荧光熄灭信号。利用这种传感能力，我们成功地将该探针应用于活的HEK293细胞内氯离子的荧光生物成像。我们还开发了另一种能够穿透细胞膜的吖啶酰胺探针，我们希望这将是一种有价值的分子工具，用于分析氯离子的生物作用。

We have developed a new acridinium amide-type fluorescent sensor for chloride anion. This probe showed a large fluorescent-off signal in response to chloride anion based on the collisional quenching mechanism in the biological relevantconcentration range (from 1 mM to 100 mM). Taking advantage of the sensing ability, we successfully applied this probe to the fluorescent bioimaging of intracellular chloride anion in living HEK293 cells.We have also developed another type of acridinium amide probe that can penetrate cell membrane, which we expect would be a valuable molecular tool for analysis of biological roles of chloride anion.