Influence of the anesthetic on nerve circuit construction of the period of growth by the visualization of the GABAergic neuron

所有程序均在成年（12-16周龄）雄性野生型（WT）和GAD 65敲除小鼠（GAD 65-/-）上进行。我们测量了从设定在53 ℃的热板上舔或跳的潜伏期。与野生型对照相比，在雄性GAD 65（-/-）中发现了反应的显著改变（p<0.01），表明增加的伤害感受。然而，在尾部浸泡试验的潜伏期没有显著差异，这是已知的脊髓介导的伤害性阈值。在福尔马林试验中，Ⅰ相和Ⅱ相的峰反应时间无显著性差异，但GAD 65（-/-）小鼠在Ⅱ相表现出明显的热敏感性延长

All procedures were performed on adult (12-16 weeks of age) male wild-type(WT) and GAD65 knockout mice (GAD65-/-).Three pain-related behavior tests(Hotplate test; Tail-immersion test; Formaline test) were performed to assess response to nociceptive stimulation. We measured latencies to licking or jumping from the hotplate set at53centigrade A significant alteration of the response was found in male GAD65 (-/-) (p<0.01)compared to their wild-type controls.indicative of increased nociceptive perception. However, there was no significant difference in the latency of the tail-immersion test, which was known to spinally-mediated nociceptive thresholds. In formalin test,there were no significant difference in peak response time of phaseI and phaseII,but GAD65(-/-)mice indicate the prolongation of hepersensitivity in phaseII