Neuroprotection using hydrogen sulfide donors : in the mice primary cultured neurons

截瘫是胸主动脉狭窄手术后的严重并发症。但其机制尚不清楚。本实验采用原代培养的小鼠脊髓神经元，研究硫化氢对脊髓缺血后神经保护作用的机制。我们使用硫化钠和GYY 4137作为硫化氢供体。但对培养神经元的保护作用较弱。利用DNA微阵列技术寻找硫化氢（Na2S和GYY 4137）作用神经元的蛋白质信号。而DNA微阵列检测结果显示，细胞凋亡率无明显变化。

Paraplegia is a severe complication after the surgery of thoracic aorta anyrism. But the mechanism is unknown. We performed a study on mechanism of the neuroprotection with hydrogen sulfide after spinal cord ischemia, using the mice primary cultured neurons. We used sodium sulphide and GYY4137 as a hydrogen sulfide donor. However, the effect of neuroprotection for cultured neurons was weak. We seek the protein signals in the neurons with hydrogen sulfide attaching (sodium sulphide and GYY4137) by DNA micro array. However, no significant change was confirmed by DNA micro array.