Assessment of non-invasive biomarkers for evaluating oocyte quality by proteomic analyses

无创性生物标志物评价卵母细胞质量可能有助于提高辅助生殖技术的成功率。鉴定优质卵母细胞的特异性生物标志物和可能参与卵泡发生的生物活性肽/蛋白。我们收集了24个卵泡液（FF）从12例接受体外受精-胚胎移植在名古屋大学医院。在所有定量检测的蛋白质中，有476个蛋白质存在于致孕卵母细胞和退化卵母细胞的FF中。18种蛋白质仅存在于来自导致妊娠的卵母细胞的FF中，9种蛋白质仅存在于来自退化的卵母细胞的FF中。利用FFs进行的蛋白质组学分析表明，FFs含有多种蛋白质。这些肽/蛋白的分析可能有助于识别与卵泡发生和卵母细胞质量有关的生物标志物。

Non-invasive biomarkers for evaluating oocyte quality might be helpful to improve success rates of assisted reproduction technology. To identify specific biomarkers of the good quality oocytes and bioactive peptides/proteins possibly involved in folliculogenesis. We collected 24 follicular fluids (FFs) from12 patients who undergone IVF-ET at Nagoya University Hospital. Proteomic analyses were performed by LC/MS. Among all quantitatively-detected proteins, 476proteins were present in both FFs derived from oocytes of resulting in pregnancy and from degenerated oocytes. 18 proteins were present only in FFs derived from oocytes resulting in pregnancy and 9 proteins were present only in FFs derived from degenerated oocytes. The proteomic analysis using FFs revealed that FFs contain many proteins. Analysis of these peptides/proteins might be helpful to identify biomarkers implicated in folliculogenesis and oocyte quality.