Expression of the stem cell makers and cell cycle in the epithelial rests of malassez

ERM细胞的基本功能和细胞增殖特性尚不清楚。在本研究中，我们研究了ERM细胞是否具有细胞生长能力和原始干细胞特征。在ERM中，细胞质中检测到p27kip1和CK-19的免疫组化染色，但GE均为阴性。ERM细胞的体外生长速率显著高于GE细胞（p<0.05）。RT-PCR结果显示，ERM细胞中端粒酶催化组分TERT的信号强于GE细胞。RT-PCR检测ERM细胞表达Nanog和Stat3多能基因。结果表明，ERM细胞是一种不寻常的细胞，表现出外胚层来源的上皮细胞的未成熟和细胞生长停滞。ERM细胞也可能具有独特的干细胞群体特性。

The essential function and cell proliferation property of the ERM cells is not well understood. In this study, we investigated whether the ERM cells have cell growth capacity and primitive stem cell characteristics.In the ERM had localization of immunohistochemical staining for p27kip1 and CK-19 were detected in cytoplasm, but GE was both negatives. Cell growth rate of the ERM cells were significantly higher than the GE cells in vitro (p<0.05). The signal of the catalytic components of telomerase, TERT, in the ERM cells was stronger than the GE cells by RT-PCR. The ERM cells were expressed pluripotent genes of Nanog and Stat3 by RT-PCR. The results suggest that The ERM cells are unusual cells that exhibit the immature and cell growth arrest of ectoderm-derived epithelial cells. The ERM cells also may have property of unique stem cell population.