Effect of periodontitis and smoking for genome methylation on IL-6 gene promoter region

传统的基因多态分析不能阐明疾病敏感基因的功能和活性。此外，人们还知道，在某些基因中，多态与蛋白质生产之间存在不一致。可能的原因是DNA甲基化修饰影响了基因信息的表达。因此，对调控基因活性的基因组甲基化的分析对于研究疾病敏感基因是必要的。本研究从4种类型的受试者(健康人、牙周炎患者、吸烟者伴/不伴牙周炎)的血液、龈沟液和牙周组织标本中提取DNA，以探讨牙周炎和吸烟对IL-6基因启动子区的影响。

Conventional gene polymorphism analysis cannot elucidate the function and activity of the disease sensitivity gene. Also, it is known that discordant exist between polymorphism and protein production in some genes. The possible cause is that the expression of gene information is influenced by DNA methylation modification. Thus, analysis of genome methylation which regulates the gene activity is necessary for research of the disease sensitivity gene. In the study, DNA extracted from blood, gingival crevicular fluid and periodontal tissues samples in the four type subjects (Healthy individuals, periodontitis patients, smoker with/without periodontitis) were used to investigate the effect of periodontitis and smoking for genome methylation on the IL-6 gene promoter region.