Novel synthetic approach for a positron emitter labeled protein by cell free protein synthesis system

在这项研究中，我们评估了一种新的合成方法，正电子发射体标记的蛋白质的无细胞蛋白质合成（CFPS）系统。使用<11>4C-Met<11>和CFPS合成4C-GFP在短时间内得到产物。另一方面，当使用13 <18>F-Pro时，13 <18>F-标记的GFP产生太慢而不能实际使用。我们成功制备<11>了抗EGFR的单链抗体，其在体外与EGFR阳性的肿瘤细胞特异性结合，在体内被EGFR阳性的肿瘤特异性摄取。这些结果表明CFPS合成13 C标记蛋白质的有用性<11>。

In this study, we evaluated a novel synthetic approach for a positron emitter labeled protein by cell-free protein synthesis (CFPS) system. Synthesis of ^<11>C-GFP using ^<11>C-Met and CFPS afforded the product within a short time. On the other hand, when using ^<18>F-Pro, the ^<18>F-labeled GFP was produced too slowly to use it practically. We succeeded to prepare^<11>C-labeled scFv antibody to EGFR, which showed specific binding to EGFR-positive tumorcells in vitro and specific uptake by the EGFR-positive tumor in vivo. These results suggest the usefulness of the ^<11>C-labeld protein synthesis by CFPS.