Challenges to replicate human norovirus with tissue cells

本项目的主要目的是为建立人诺如病毒对组织细胞的感染获得宝贵的知识。我们成功构建了含有绿色荧光蛋白（GFP-NoVLP）的诺如病毒样颗粒。应用GFP-NoVLP确认人诺如病毒在组织细胞中的侵入和脱壳，但发现荧光量不足以观察GFP-NoVLP在组织细胞中的运动。因此，我们关注组织细胞对病毒感染的细胞反应，并试图寻找可用于检测病毒感染的遗传标记。以1型脊髓灰质炎病毒（PV1） Sabin株为试验病毒，对感染PV1的INT407细胞进行转录组分析。因此，KCNJ4基因是检测病毒感染的最有希望的遗传标记。

The main purpose of this project was to acquire precious knowledge for establishing the infection of human norovirus to tissue cells. We successfully constructed a norovirus-like particle with green fluorescence protein (GFP-NoVLP). GFP-NoVLP was applied for confirming the intrusion and decapsidation of human norovirus in tissue cells, but it was found that the amount of fluorescence was not enough to observe the movement of GFP-NoVLP in tissue cells. Therefore, we paid attentions to cellular responses of tissue cells against the virus infection, and tried to identify genetic markers available for the detection of virus infection. Poliovirus type 1 (PV1) Sabin strain was used as a test virus, and a transcriptome analysis of INT407 cells infected with PV1 was conducted. As a result, KCNJ4 gene, responsible for potassium homeostasis, was found as the most promising genetic marker for the detection of virus infection.