Development of targeted cell therapeutic systems based on cell characteristics

为了实现细胞治疗，我们尝试开发“基于细胞特性的靶向细胞治疗系统”。我们研究了使用多细胞球体作为体内应用后最大化细胞存活和功能的方法。使用聚二甲基硅氧烷微囊可以得到大小可控的球体，但细胞与微囊表面的黏附阻止了某些类型的细胞形成球体。用聚N-异丙基丙烯酰胺包覆微囊解决了这个问题，我们用所有类型的细胞成功地制备了多细胞球体。然后，使用产生胰岛素的NIT-1细胞在糖尿病模型小鼠中检验多细胞球体的治疗潜力。与NIT-1细胞悬液相比，NIT-1球体显示出延长小鼠存活时间和降低血糖水平的高活性。

To realized cell therapy, we tried to develop “targeted cell therapeutic systems based on cell characteristics”. We examined the use of multicellular spheroids as a method to maximize the cell survival and functions after in vivo application. Spheroids with controlled size were obtained by using polydimethylsiloxane-based micro-pockets, but cell adhesion to the micro-pocket surface prevented some types of cells from spheroid formation. Coating the micro-pockets with poly(N-isopropylacrylamide) solved this problem, and multicellular spheroids we re successfully prepared using all the cell types used. Then, therapeutic potential of multicellular spheroids was examined in diabetic model mice using insulin-producing NIT -1 cells. NIT-1 spheroids showed prolonged survival of the mice and high activity to reduce blood glucose level compared with NIT -1 cell suspension.