Elucidation of molecular mechanisms involved in the immunomodulatory function of newly developed adipose tissue derived mesenchymal stem cells

低血清培养的脂肪间充质干细胞（adipose derived mesenchymal stem cells，LASC）可抑制T细胞增殖，诱导巨噬细胞向M2型分化，其作用强于高血清培养的脂肪间充质干细胞（adipose derived mesenchymal stem cells，HASC）和骨髓间充质干细胞（bone marrow derived mesenchymal stem cells，BM-MSC）。本研究通过与HASC和BM-MSC的比较，探讨LASC免疫抑制作用的机制。通过中和实验和刺激实验发现，LASC来源的PGE 2和IL-6是M2巨噬细胞极化所必需的，PGE 2通过EP 4受体发挥作用。

We have proven that low serum cultured adipose derived mesenchymal stem cells (LASC) suppress T cell proliferation and induce polarization of macrophages into immunomodulatory M2 type, and that these effects of LASC were much stronger than high serum cultured adipose derived mesenchymal stem cells (HASC) or bone marrow derived mesenchymal stem cells (BM-MSC). Inthis study, we studied the mechanisms involved in the immunosuppressive functionof LASC by comparing with HASC or BM-MSC. We found by neutralization experiment and stimulation experiment that LASC derived PGE2 and IL-6 are necessary for the polarization of M2 macrophages, and that PGE2 function via EP4 receptor.