Elucidation of the role of syndecans in infectious or inflammatory diseases of ocular surface

实验分为三种：培养人角膜或结膜上皮细胞片实验、syndecan-4基因敲除小鼠实验和临床样本实验。用培养细胞片评价。在人原代培养的角膜或结膜细胞片和人sv40永动角膜细胞片中，Syndecan-4被il -1b显著增加。动物实验。比较sydecan-4基因敲除小鼠和野生型小鼠角膜创面愈合速度。两种小鼠物理创伤模型的角膜创面愈合速度相似。两种小鼠炎症性创面模型（碱烧伤和角膜基质注射LPS）的角膜创面愈合速度相似。与syndecan-4异基因敲除小鼠相比，syndecan-4同源敲除小鼠的血管新生和淋巴管新生增加。临床样品评价。测定了眼表疾病患者和健康志愿者泪液中syndecan-4的浓度。男性52例，女性84例。平均年龄66±18岁。泪中syndecan-4的平均浓度为3485±3537 pg/ml。活动性眼表炎症患者泪液中syndecan-4的平均浓度为2965±4497 pg/ml。

Three kinds of experiments were performed: experiments using cultured human corneal or conjunctival epithelial cell sheets, experiments using syndecan-4 knockout mice, and experiments using clinical samples.Evaluation using cultured cell sheet. Syndecan-4 was significantly increased byIL-1b in human primary cultured corneal or conjunctival cell sheets and humanSV40-immortarized corneal cell sheets.Animal experiments. The speed of wound healing in the cornea was compared in sydecan-4 knockout mice and wild type mice. The speed of corneal wound healing in the physical wound model was similar in the two kinds of mice. The speed ofcorneal wound healing in the inflammatory wound model (alkali burn and LPS injection into corneal stroma) was similar in the two kinds of mice as well. Angiongenesis and lymphangiogenesis were increased in syndecan-4 homo-knockout mice compared to syndecan-4 hetero-knockout mice.Evaluation on clinical samples. The concentration of syndecan-4 in the tear was measured in patients with ocular surface diseases and healthy volunteers. Tear was collected in 52 male and 84 female. The average age was 66 ± 18 years. The average concentration of syndecan-4 in the tear was 3485 ± 3537 pg/ml. The average concentration of syndecan-4 in the tear in patients with active ocular surfaceinflammation was 2965 ± 4497 pg/ml.