Investigation of nerve regeneration and its plasticity using a new antibodies against phosphorylated GAP-43 (p-GAP-43)

观察端侧神经吻合后周围神经的组织学变化，以确定手术中神经周围开放和故意供体神经损伤的影响。在肌皮神经（受体）与尺神经（供体）接合7天后，使用抗神经丝- h （RT97）和磷酸化的GAP-43 （p-GAP-43）抗体对神经进行免疫组织化学分析。后者被发现专门检测生成的神经纤维。统计分析显示，端侧神经吻合时，神经周围开放和供体神经故意损伤的疗效增加，提示部分神经纤维突出伴部分轴突吻合或神经切断术对端侧神经吻合时有效的轴突再生很重要。

Histological changes were observed in peripheral nerves following end-to-side nerve coaptation to determine the effects of perineurial opening and deliberate donor nerve injury during surgery. Seven days after coaptation of the musculocutaneous (recipient) nerve to the ulnar (donor) nerve, the nerves were immunohistochemically analysed using antibodies against neurofilament-H (RT97) and phosphorylated GAP-43 (p-GAP-43). The latter was found to detect specifically generating nerve fibers. Statistical evaluation revealed increased efficacy of perineurial opening and deliberate donor nerve injury in end-to-side nerve coaptation, suggesting that partial nerve fiber herniation with partial axonotmesis or neurotomesis was important for effective axonal regeneration in end-to-side nerve coaptation.