Dynamics of polarisome and ROS generating complex during pathogenic development of fungi

本研究探讨了NADPH氧化酶（Nox）在渗透宿主过程中的作用机制。在黑斑病中，存在两种Nox同系物，NoxA和NoxB，并且只有NoxB参与宿主渗透。因此，我们评估了Nox(s)的表达模式和定位。在孢子萌发过程中诱导Nox的表达。NoxB蛋白定位于附着胞。noxB的破坏导致附着胞肌动蛋白积累紊乱。此外，NoxB与RacA相互作用。断裂突变体影响了菌丝的多个生长阶段，丧失了致病性。

This study was evaluated the functional mechanism of NADPH oxidase (Nox) during host penetration. In black spot disease, two Nox homologues, NoxA and NoxB are present and only the NoxB was involved in host penetration. Therefore, we evaluated the expression pattern and localization of Nox(s). The expression of Nox were induced during spore germination. NoxB protein was localized at the appressorium. The disruption of noxB resulted in disorder of actin accumulation at the appressorium. Moreover, NoxB was interacted with RacA. The disruption mutant of racA was affected multiple hyphal growth stages and lost pathogenicity.