Basic characteristics of urinary insecticide and its metabolite levels in Japanese

在日本，2007年可耕种和永久耕种的农药消费量估计为1.2吨/平方公里，远远高于其他经合组织国家。为了弄清日本尿中杀虫剂及其代谢物含量的特点，建立了一种简便、准确、高通量的检测一次性尿布中拟除虫菊酯代谢物CDCA和3-PBA的方法。建立了人尿中7种新烟碱及其代谢物的GC-MS和LC-MS/MS快速分析方法。这些方法不仅可用于成人，也可用于对与杀虫剂接触有关的尿液生物标记物进行常规生物监测。采用交叉验证法和室内质控法(G-EQUAS51)对方法进行验证。我们认为，某些生物标志物的水平取决于采样季节、年龄和农药使用情况。

In Japan, the consumption of pesticides was estimated to be 1.2 ton/km2 of arable and permanent cropland in 2007, which was much higher than those of other OECD countries. The aim of this study is to clarify the characteristics of urinary insecticide and its metabolite levels in Japanese.We developed a simple, precise, and high-throughput method for the detection of urinary pyrethroid metabolites CDCA and 3-PBA extracted from disposable diapers. Moreover, a rapid and sensitive analytical method using GC-MS and LC-MS/MS was developed for the measurement of 7 neonicotinoids (NEO) and its metabolites from human urine. These methods can be applied to routine biological monitoring of urinary biomarkers associated with insecticide exposure not only in adults but young children. These methods were validated by cross validation assay and external quality control assay (G-EQUAS 51). We suggested that the levels of some biomarkers were depends on sampling season, age and pesticide usage.