Microfluidic device for cellular assay of various samples

通过使用微型销机械地描述侧壁而具有可移动侧壁的可重构微流体通道在通道内输送难以流动的物体方面具有优势，并且因此其可用于分析各种分析物/样品。然而，该装置在多次供给销和将通道中的流体吸入间隙中方面存在问题。我们开发了一种探头，可以将单个引脚加热到高温，并通过摩擦给它们提供动力，以及一种密封剂，其粘度随温度急剧变化。我们发现聚烯烃与二氧化硅或氟树脂填料的混合物降低了由热引起的粘度下降，而它仅显著降低了加热销附近的粘度。我们还成功地在一个通道内将不同丙烯酰胺配方的多个凝胶块成一行图案化。

Reconfigurable microfluidic channel with movable sidewalls by mechanically descritizing the sidewalls using miniature pins has advantages in the transporting difficult-to-flow objects within the channels, and thus it is useful in analyzing various analytes/samples. The device, however, has problems in multiple feeding of the pins, and suctioning the fluid in the channel into the gaps. We have developed a probe that heats single pins to an elevated temperature and that feeds them with friction, and a sealant that changes its viscosity steeply with the temperature. We found a mixure of polyolefins with silica or fluororesin fillers reduced the viscosity drop by heat while it significantly lower the viscosity only near the heated pin. We also successfully patterned multiple gel blocks of different formulation of acrylamide in line within one channel.