Development of optically-driven tool for nano-bio processing and its applications in molecular dissection of genomic DNA

在本研究项目中，我们开发了一种新型的纳米/微米加工工具，用于加工单个生物分子，用于基因组分析领域的研究。发展了纳米/微米结构的制造技术，使我们能够在荧光显微镜下控制结构的可见性。对它们的特性进行了评价，包括制作成品率和捕获力。我们还通过表面等离子体共振传感验证了蛋白质在其表面的固定化。结果表明，蛋白质修饰纳米/微米工具的蛋白质固定化和功能化是成功的。

In this research project, we developed a novel nano/micro-fabricated tool for processing single biomolecule for the application in the research field of genome analysis. The technique for fabricating nano/micro structures was developed, which enables us to control the visibility of the structures under a fluorescence microscope. Their characteristics including the yield of fabrication and the trapping force were evaluated. We also verified the immobilization of proteins on their surface by surface plasmon resonance sensing. The results suggest the successful protein immobilization and the functionalization for a protein-modified nano/micro tool.