Electrophysiological recording using Spatially Arranged Microelectrode Embedded into 3D Culture

3D培养更有可能模拟生理组织环境。本研究的重点是利用嵌入三维培养物的空间排列微电极在三维培养物中进行空间细胞记录。线焊接?基于探针技术使得提供三维微电极成为可能。我们证明了使用嵌入的微电极可以成功记录3D神经元培养物中的电生理活动。我们还发现，细胞操作、光学传感和电化学传感与空间排列的微电极相结合。

3D cultures are more likely to mimic physiological tissue environments. This research focuses on spatialextracellular recordings inside 3D cultures using spatially arranged microelectrode embedded into 3Dcultures. Wire?bonding?based probe technology makes it possible to provide the 3D microelectrodes.We demonstrated electrophysiological activities inside 3D neuronal cultures could be successfullyrecorded using the embedded microelectrodes. We also indicated that cell manipulation, optical sensingand electrochemical sensing were integrated with the spatially arranged microelectrode.