Identification and function analysis of cancer stem cell in esophageal cancer

纤维蛋白原是影响食管癌预后的因素之一。在食管鳞癌细胞系中，整合素β 3（CD 61/纤维蛋白原受体）阳性细胞系（TE 8）在含有生长因子（EGF和bFGF）的无血清培养基中显示球形形成。提示CD 61阳性细胞具有肿瘤干细胞自我更新的特性。细胞形态学功能分析显示，CD 61阳性细胞具有较强的趋化能力。另一方面，在食管鳞状细胞癌细胞系TE 10和TE 11中富集癌症干细胞，随后通过长期暴露于抗癌剂（5 FU）来纯化癌症干细胞。6个月后，两种细胞均获得了耐药能力，检测miRNAs（miR-200家族或miR-let-7家族）的表达变化。

Fibrinogen is one of the prognostic factors in esophageal cancer. In esophageal squamous carcinoma cell lines, integrin beta 3 (CD61/fibrinogen receptor) positive cell line (TE8) showed sphere-formation in serum-free medium containing growth factors (EGF and bFGF). This result suggested that CD61- positive cells have the property of self-renewal of cancer stem cell. Furthermore, the analysis of cell morphology functions, CD61-positive cells indicated the high chemotactic ability. On the other hand, to enrich for cancer stem cells, followed by purification of cancer stem cells by long-term exposure to anti-cancer agents (5FU) in esophageal squamous carcinoma cell lines TE10 and TE11. After six months, both cells have acquired the ability for drug resistance, and the expression variation of miRNAs (miR-200 family or miR-let-7 family) were detected.