Development of intraoperative navigation system for robot-assisted radical prostatectomy utilizing two-photon microscope

本研究旨在分析双光子显微镜在机器人辅助根治性乳腺癌术中导航系统中应用的可行性。观察并处死雄性大鼠，采用HE染色、Masson三色染色、抗甲状腺素羟化酶免疫组化染色、抗nNOS免疫组化染色。虽然很难设定合适的波长来观察大鼠盆腔的自主神经，但我们从本研究中获得了一些想法，并报告了本研究中有关盆腔器官神经解剖和术中成像系统的一些观察结果。

The aim of the present study was to analyze a feasibility of utilizing two-photon microscope in the intraoperative navigation system for robot-assisted radical prostatectomy. Male rats were observed and sacrificed, then the tissues were stained using H-E staining, Masson-Trichrome staining, anti-thyroxin Hydroxidase immunostaining, and anti-nNOS immunostaining. Although it was difficult to set suitable wavelength to observe the autonomic nerves in the rat pelvis, we obtained ideas from the present study and reported some observations about neuroanatomy for pelvic organs and intraoperative imaging systems derived from the present study.