Dual, realtime minitoring of Ca2+ and ROS signals in plant cells

活性氧(ROS)在细胞内/细胞间信号转导中起着关键作用。最近的研究表明，钙信号和ROS信号通过产生[Ca~(2+)]_c振荡和发挥未知的其他关键作用，在精确的细胞内信号转导中相互作用。在本研究中，我们试图建立一种新的方法，利用对钙离子或ROS敏感的光蛋白和发光光谱来同时监测[ROS]c和[Ca2+]c，以阐明植物中ROS信号的生理作用和机制，以及它们与Ca2+信号的相互作用。

Reactive oxygen species (ROS) play key roles in intra- / inter-cellular signal transduction. Recent studies revealed that Ca2+-signal and ROS-signal are interplaying for the precise intracellular signal transduction through generating [Ca2+]c oscillation and playing the other key roles of still unknown. In the present study, we tried to develop a novel method to monitor [ROS]c and [Ca2+]c simultaneously using Ca2+- or ROS-sensitive photoproteins and spectrometry of luminescence to clarify the physiological roles and mechanisms of ROS-signal and their interplay with Ca2+-signal in plants.