Molecular mechanisms of wound-triggered cell dedifferentiation

WIND1是拟南芥创面诱导因子，对细胞去分化和愈伤组织形成具有正向调控作用。酵母1杂交分析表明，一个与热休克（HS）相关的转录因子是wind1上游因子的候选因子之一。该因子不仅因热休克而上调，也因创伤而上调。当植物处于热胁迫条件下，WIND1表达上调，植物更易形成愈伤组织。WIND1功能的丧失抑制了这种愈伤组织的诱导，表明WIND1参与了热诱导细胞的去分化。在正常和热胁迫条件下，hs相关转录因子功能缺失突变体中WIND1的表达水平均小于野生型植物。这些结果和其他观察结果阐明了hs触发的细胞去分化级联，该级联是由hs相关因子和WIND1介导的。

WIND1 is wound inducible transcription factor which positively regulates cell dedifferentiation and callus formation at wound site of Arabidopsis. Yeast one hybrid analysis revealed that a heat shock (HS)-related transcription factor is one candidates of WIND1-upstream factor. This factor is upregulated by not only heat shock but also by wound. When plants are treated on heat-stress condition, WIND1 expression is upregulated and plant comes to form callus easily. WIND1 loss of function suppresses this callus induction, suggesting WIND1 participates in heat inducible cell dedifferentiation. Level of WIND1 expression in the loss-of-function mutant of the HS-related transcription factor is smaller than that of wild-type plants under both normal and heat stress condition. These results and other observations clarified that there is HS-triggered cell dedifferentiation cascade, which is mediated by HS-related factor and WIND1.