Identification of torque generation part in flagellar motor by using fluorescence polarization

研制了一种用于检测荧光分子偏振的显微系统。通过使用该显微系统，检测来自单个GFP分子的荧光偏振，并且根据GFP在P偏振和S偏振中的荧光强度来估计GFP分子在盖玻片表面上的方向。构建了同时观察GFP融合蛋白荧光和鞭毛马达旋转的显微系统。通过使用该显微镜系统，通过同时可视化用GFP标记的CheY和活细胞中马达的旋转开关，证明马达的旋转开关直接由磷酸化CheY的结合和解离调节。这项研究的结果发表在Sci。信号了2014. 7：ra32.

The microscopic system to detect the polarization of fluorescent molecules was develoved. By using this microscopic system, the polarization of fluorescence from a single GFP molecule was detected, and the direction of a GFP molecule on the surface of coverslip was estimated from the fluorescent intensities of GFP in P-polarization and S-polarization. The microscopic system to simultaneously observe the fluorescence from GFP-fusion proteins and the rotation of flagellar motor was also constructed. By using this microscopic system, it was demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of phosphorylated CheY by simultaneously visualizing CheY tagged with GFP and the rotational switching of a motor in live cells. The results of this study was published from Sci. Signal. 2014. 7: ra32.