Structural and functional diversities of mammalian cilia

1)我们研究了哺乳动物细胞中纤毛数量调节的分子机制。基于转录组分析和随后的敲除实验，我们确定了一个新的感兴趣的基因。我们已经构建了CRISPR/Cas9质粒以产生该基因的敲除小鼠。2)为了监测多纤毛发生的细胞事件，我们已经产生了慢病毒结构，通过荧光蛋白标记睫状体和基体。3)我们已经调查了独特的功能过渡脉络丛上皮细胞（CPECs）从产前时期的纤毛。4)我们分析了TRPV 4在CPEC中的纤毛定位和功能意义。

1) We have investigated the molecular mechanism of ciliary number regulation in mammalian cells. Based on transciptome analysis and the following knockdown experiments, we identified one novel gene of interest. We have constructed CRISPR/Cas9 plasmid to generate knockout mouse of this gene. 2) To monitor the cellular event of multiciliogenesis, we have generated lentiviral constructs to label the ciliary shaft and the basal body by fluorescent proteins. 3) We have investigated the unique functional transition of cilia in choroid plexus epithelial cells (CPECs) from the prenatal period. 4) We have analyzed the ciliary localization and functional significance of TRPV4 in CPECs.