Analyses of nuclear envelope dynamics using a novel model fission yeast

为了遗传遗传信息，围绕DNA的核膜必须正确地遗传给子代细胞。高等真核细胞与人类一样，具有开放的有丝分裂系统，伴随着核膜的破裂和再生。另一方面，低等真核细胞如酵母具有封闭的有丝分裂系统，并伴有核膜的收缩。到目前为止，这种差异的意义和机制还不清楚。在我们的研究中，使用了一种具有‘半开放’有丝分裂的分裂酵母。通过遗传筛选寻找与核膜遗传相关的基因，发现APC/环体和脂肪酸合成酶在核膜遗传中起作用。由于APC/环体是蛋白质降解途径中的关键因子，而脂肪酸是构建膜的重要因素，我们提出了一个通过蛋白质降解来调节核膜水平的模型，这是DNA遗传所必需的。

For the inheritance of genetical information, a nuclear membrane, surrounding DNA, has to be inherited to daughter cells correctly. Higher eukariotic cells like human have 'open' mitosis system accompanying breakdown and regeneration of nuclear membrane. On the other hand, lower eukariotic cells like yeasts have 'closed' mitosis system accompanying shrinkage of nuclear membrane. The significance and mechanism of the difference has not been understood so far.For our study, a fission yeast S. japonicus, which has 'semi-open' mitosis, was used. Searching genes relating to inheritance of nuclear membrane by genetical screening, it was found that APC/Cyclosome and fatty acid synthase function for the inheritance of nuclear membrane. Because APC/Cyclosome is a key factor in the protein degradation pathway, and fatty acids are important for constructing membrane, we proposed a model that regulation of level of nuclear membrane via protein degradation is necessary for the inheritance of DNA.