Epigenetic regulation of key developmental genes from embryo to adult

我们进行了青鳉鱼多能囊胚胚胎和成体肌节和肝脏的表观基因组分析。 我们发现，在发育基因位点中，低甲基化DNA结构域（HMD）的大小与囊胚发育基因表达的抑制水平呈正相关，当邻近基因在成体组织中活跃表达时，HMD会缩短。与人ES细胞、斑马鱼胚胎和成体肌肉的比较分析表明，HMD大小与发育基因抑制水平之间的相关性是保守的。因此，本研究突出了从多能胚胎到成体由表观遗传结构域大小介导的发育基因调控的新特征。

We performed the epigenomic analysis of pluripotent blastula embryos and adult myotome and liver in medaka fish. We revealed that, in developmental gene loci, the size of hypomethylated DNA domain (HMD) positively correlates with the repression levels of developmental gene expression in blastula, and HMDs become shortened when the adjacent gene is actively expressed in adult tissues. The comparative analyses with human ES cells, zebrafish embryos and adult muscle showed the conservation of the correlation between HMD size and the repression levels of developmental genes. Therefore, this study highlights the novel features of developmental gene regulation mediated by the size of epigenetic domain from pluripotent embryos to adult.